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cd15 cd14 cd11b cd33 populations  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd15 cd14 cd11b cd33 populations
    <t>CD14+HLA-DR−/low</t> MDSC are increased in patients with GI cancer compared to controls, and this increase is consistent across each method of processing. a Flow cytometry analysis of both fresh and cryopreserved PBMC and whole blood which has undergone red cell lysis in patients with advanced GI cancer (n = 41) and healthy subjects (n = 44). Relative frequency is shown in a while b illustrates the data calculated for estimated absolute number; c representative dot plots of CD14+HLA-DR−/low MDSC in fresh PBMC, cryopreserved PBMC or whole blood; d gating strategy; e gross morphology on cytospin
    Cd15 Cd14 Cd11b Cd33 Populations, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd15 cd14 cd11b cd33 populations/product/Miltenyi Biotec
    Average 99 stars, based on 17 article reviews
    cd15 cd14 cd11b cd33 populations - by Bioz Stars, 2026-03
    99/100 stars

    Images

    1) Product Images from "Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies"

    Article Title: Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies

    Journal: Cancer immunology, immunotherapy : CII

    doi: 10.1007/s00262-012-1332-3

    CD14+HLA-DR−/low MDSC are increased in patients with GI cancer compared to controls, and this increase is consistent across each method of processing. a Flow cytometry analysis of both fresh and cryopreserved PBMC and whole blood which has undergone red cell lysis in patients with advanced GI cancer (n = 41) and healthy subjects (n = 44). Relative frequency is shown in a while b illustrates the data calculated for estimated absolute number; c representative dot plots of CD14+HLA-DR−/low MDSC in fresh PBMC, cryopreserved PBMC or whole blood; d gating strategy; e gross morphology on cytospin
    Figure Legend Snippet: CD14+HLA-DR−/low MDSC are increased in patients with GI cancer compared to controls, and this increase is consistent across each method of processing. a Flow cytometry analysis of both fresh and cryopreserved PBMC and whole blood which has undergone red cell lysis in patients with advanced GI cancer (n = 41) and healthy subjects (n = 44). Relative frequency is shown in a while b illustrates the data calculated for estimated absolute number; c representative dot plots of CD14+HLA-DR−/low MDSC in fresh PBMC, cryopreserved PBMC or whole blood; d gating strategy; e gross morphology on cytospin

    Techniques Used: Flow Cytometry, Lysis

    CD15+CD14− cells but not CD15+CD14−CD11b+CD33+ are increased in patients with GI cancer compared to controls. Flow cytometry analysis of both fresh and cryopreserved PBMC and whole blood which has undergone red cell lysis in patients with advanced GI cancer (n = 41) and healthy subjects (n = 44). a Relative frequency CD15+CD14−;b estimated absolute number CD15+CD14 -; c relative frequency CD15+CD14−CD11b+CD33+; d estimated absolute number CD15+CD14−CD11b+CD33+; e representative dot plots of CD15+CD14−CD11b+CD33+ in fresh PBMC, cryopreserved PBMC or whole blood; f gross morphology on cytospin
    Figure Legend Snippet: CD15+CD14− cells but not CD15+CD14−CD11b+CD33+ are increased in patients with GI cancer compared to controls. Flow cytometry analysis of both fresh and cryopreserved PBMC and whole blood which has undergone red cell lysis in patients with advanced GI cancer (n = 41) and healthy subjects (n = 44). a Relative frequency CD15+CD14−;b estimated absolute number CD15+CD14 -; c relative frequency CD15+CD14−CD11b+CD33+; d estimated absolute number CD15+CD14−CD11b+CD33+; e representative dot plots of CD15+CD14−CD11b+CD33+ in fresh PBMC, cryopreserved PBMC or whole blood; f gross morphology on cytospin

    Techniques Used: Flow Cytometry, Lysis

    Analysis of CD14+HLA-DR−/low MDSC in patients with GI cancer. CD14+HLA-DR−/low, CD14+HLA-DR+, and CD15+CD14−CD11b+CD33+ cells were cocultured at different ratios with autologous anti-CD3/CD28−cells. Supernatant was taken from the cultures before addition of 3H-thymidine and measured for IFN-γ by ELISA. Results shown are representative of 3 independent experiments
    Figure Legend Snippet: Analysis of CD14+HLA-DR−/low MDSC in patients with GI cancer. CD14+HLA-DR−/low, CD14+HLA-DR+, and CD15+CD14−CD11b+CD33+ cells were cocultured at different ratios with autologous anti-CD3/CD28−cells. Supernatant was taken from the cultures before addition of 3H-thymidine and measured for IFN-γ by ELISA. Results shown are representative of 3 independent experiments

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Correlations CD14+HLA-DR−/low MDSC across method of processing. a Fresh versus cryopreserved PBMC; b PBMC versus whole blood lysis; c whether absolute number or relative frequency calculated
    Figure Legend Snippet: Correlations CD14+HLA-DR−/low MDSC across method of processing. a Fresh versus cryopreserved PBMC; b PBMC versus whole blood lysis; c whether absolute number or relative frequency calculated

    Techniques Used: Lysis



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    cd15 cd14 cd11b cd33 populations  (Miltenyi Biotec)
    99
    Miltenyi Biotec cd15 cd14 cd11b cd33 populations
    <t>CD14+HLA-DR−/low</t> MDSC are increased in patients with GI cancer compared to controls, and this increase is consistent across each method of processing. a Flow cytometry analysis of both fresh and cryopreserved PBMC and whole blood which has undergone red cell lysis in patients with advanced GI cancer (n = 41) and healthy subjects (n = 44). Relative frequency is shown in a while b illustrates the data calculated for estimated absolute number; c representative dot plots of CD14+HLA-DR−/low MDSC in fresh PBMC, cryopreserved PBMC or whole blood; d gating strategy; e gross morphology on cytospin
    Cd15 Cd14 Cd11b Cd33 Populations, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd15 cd14 cd11b cd33 populations/product/Miltenyi Biotec
    Average 99 stars, based on 1 article reviews
    cd15 cd14 cd11b cd33 populations - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    CD14+HLA-DR−/low MDSC are increased in patients with GI cancer compared to controls, and this increase is consistent across each method of processing. a Flow cytometry analysis of both fresh and cryopreserved PBMC and whole blood which has undergone red cell lysis in patients with advanced GI cancer (n = 41) and healthy subjects (n = 44). Relative frequency is shown in a while b illustrates the data calculated for estimated absolute number; c representative dot plots of CD14+HLA-DR−/low MDSC in fresh PBMC, cryopreserved PBMC or whole blood; d gating strategy; e gross morphology on cytospin

    Journal: Cancer immunology, immunotherapy : CII

    Article Title: Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies

    doi: 10.1007/s00262-012-1332-3

    Figure Lengend Snippet: CD14+HLA-DR−/low MDSC are increased in patients with GI cancer compared to controls, and this increase is consistent across each method of processing. a Flow cytometry analysis of both fresh and cryopreserved PBMC and whole blood which has undergone red cell lysis in patients with advanced GI cancer (n = 41) and healthy subjects (n = 44). Relative frequency is shown in a while b illustrates the data calculated for estimated absolute number; c representative dot plots of CD14+HLA-DR−/low MDSC in fresh PBMC, cryopreserved PBMC or whole blood; d gating strategy; e gross morphology on cytospin

    Article Snippet: For functional analysis, CD14 + HLA-DR −/low , CD14 + HLA-DR + , CD15 + CD14 − CD11b + CD33 + , and CD15 + CD14 − CD11b + CD33 − populations were incubated at different ratios with CD3/CD28 (Miltenyi Biotech)-stimulated CD8 T cells.

    Techniques: Flow Cytometry, Lysis

    CD15+CD14− cells but not CD15+CD14−CD11b+CD33+ are increased in patients with GI cancer compared to controls. Flow cytometry analysis of both fresh and cryopreserved PBMC and whole blood which has undergone red cell lysis in patients with advanced GI cancer (n = 41) and healthy subjects (n = 44). a Relative frequency CD15+CD14−;b estimated absolute number CD15+CD14 -; c relative frequency CD15+CD14−CD11b+CD33+; d estimated absolute number CD15+CD14−CD11b+CD33+; e representative dot plots of CD15+CD14−CD11b+CD33+ in fresh PBMC, cryopreserved PBMC or whole blood; f gross morphology on cytospin

    Journal: Cancer immunology, immunotherapy : CII

    Article Title: Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies

    doi: 10.1007/s00262-012-1332-3

    Figure Lengend Snippet: CD15+CD14− cells but not CD15+CD14−CD11b+CD33+ are increased in patients with GI cancer compared to controls. Flow cytometry analysis of both fresh and cryopreserved PBMC and whole blood which has undergone red cell lysis in patients with advanced GI cancer (n = 41) and healthy subjects (n = 44). a Relative frequency CD15+CD14−;b estimated absolute number CD15+CD14 -; c relative frequency CD15+CD14−CD11b+CD33+; d estimated absolute number CD15+CD14−CD11b+CD33+; e representative dot plots of CD15+CD14−CD11b+CD33+ in fresh PBMC, cryopreserved PBMC or whole blood; f gross morphology on cytospin

    Article Snippet: For functional analysis, CD14 + HLA-DR −/low , CD14 + HLA-DR + , CD15 + CD14 − CD11b + CD33 + , and CD15 + CD14 − CD11b + CD33 − populations were incubated at different ratios with CD3/CD28 (Miltenyi Biotech)-stimulated CD8 T cells.

    Techniques: Flow Cytometry, Lysis

    Analysis of CD14+HLA-DR−/low MDSC in patients with GI cancer. CD14+HLA-DR−/low, CD14+HLA-DR+, and CD15+CD14−CD11b+CD33+ cells were cocultured at different ratios with autologous anti-CD3/CD28−cells. Supernatant was taken from the cultures before addition of 3H-thymidine and measured for IFN-γ by ELISA. Results shown are representative of 3 independent experiments

    Journal: Cancer immunology, immunotherapy : CII

    Article Title: Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies

    doi: 10.1007/s00262-012-1332-3

    Figure Lengend Snippet: Analysis of CD14+HLA-DR−/low MDSC in patients with GI cancer. CD14+HLA-DR−/low, CD14+HLA-DR+, and CD15+CD14−CD11b+CD33+ cells were cocultured at different ratios with autologous anti-CD3/CD28−cells. Supernatant was taken from the cultures before addition of 3H-thymidine and measured for IFN-γ by ELISA. Results shown are representative of 3 independent experiments

    Article Snippet: For functional analysis, CD14 + HLA-DR −/low , CD14 + HLA-DR + , CD15 + CD14 − CD11b + CD33 + , and CD15 + CD14 − CD11b + CD33 − populations were incubated at different ratios with CD3/CD28 (Miltenyi Biotech)-stimulated CD8 T cells.

    Techniques: Enzyme-linked Immunosorbent Assay

    Correlations CD14+HLA-DR−/low MDSC across method of processing. a Fresh versus cryopreserved PBMC; b PBMC versus whole blood lysis; c whether absolute number or relative frequency calculated

    Journal: Cancer immunology, immunotherapy : CII

    Article Title: Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies

    doi: 10.1007/s00262-012-1332-3

    Figure Lengend Snippet: Correlations CD14+HLA-DR−/low MDSC across method of processing. a Fresh versus cryopreserved PBMC; b PBMC versus whole blood lysis; c whether absolute number or relative frequency calculated

    Article Snippet: For functional analysis, CD14 + HLA-DR −/low , CD14 + HLA-DR + , CD15 + CD14 − CD11b + CD33 + , and CD15 + CD14 − CD11b + CD33 − populations were incubated at different ratios with CD3/CD28 (Miltenyi Biotech)-stimulated CD8 T cells.

    Techniques: Lysis